Immunization with a multisubunit vaccine considerably reduces establishment of infective larvae in a rodent model of Brugia malayi.
Identifieur interne : 003576 ( Main/Exploration ); précédent : 003575; suivant : 003577Immunization with a multisubunit vaccine considerably reduces establishment of infective larvae in a rodent model of Brugia malayi.
Auteurs : Nidhi Shrivastava [Inde] ; Prashant Kumar Singh ; Jeetendra Kumar Nag ; Susheela Kushwaha ; Shailja Misra-BhattacharyaSource :
- Comparative immunology, microbiology and infectious diseases [ 1878-1667 ] ; 2013.
Descripteurs français
- KwdFr :
- Activation des lymphocytes (immunologie), Animaux, Anticorps antihelminthe (immunologie), Antigènes d'helminthe (immunologie), Brugia malayi (immunologie), Cytokines (biosynthèse), Espèces réactives de l'oxygène (métabolisme), Filariose lymphatique (), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Immunisation, Immunoglobuline G (immunologie), Immunoglobuline M (immunologie), Larve (immunologie), Murinae (parasitologie), Protéines de protozoaire (administration et posologie), Protéines de protozoaire (immunologie), Protéines recombinantes (administration et posologie), Protéines recombinantes (immunologie), Vaccins antiprotozoaires (administration et posologie), Vaccins antiprotozoaires (immunologie), Vaccins sous-unitaires (administration et posologie), Vaccins sous-unitaires (immunologie), Vaccins synthétiques (administration et posologie), Vaccins synthétiques (immunologie).
- MESH :
- administration et posologie : Protéines de protozoaire, Protéines recombinantes, Vaccins antiprotozoaires, Vaccins sous-unitaires, Vaccins synthétiques.
- biosynthèse : Cytokines.
- immunologie : Activation des lymphocytes, Anticorps antihelminthe, Antigènes d'helminthe, Brugia malayi, Filariose lymphatique, Immunoglobuline G, Immunoglobuline M, Larve, Protéines de protozoaire, Protéines recombinantes, Vaccins antiprotozoaires, Vaccins sous-unitaires, Vaccins synthétiques.
- métabolisme : Espèces réactives de l'oxygène.
- parasitologie : Filariose lymphatique, Murinae.
- Animaux, Filariose lymphatique, Immunisation.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (immunology), Antigens, Helminth (immunology), Brugia malayi (immunology), Cytokines (biosynthesis), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Elephantiasis, Filarial (prevention & control), Immunization, Immunoglobulin G (immunology), Immunoglobulin M (immunology), Larva (immunology), Lymphocyte Activation (immunology), Murinae (parasitology), Protozoan Proteins (administration & dosage), Protozoan Proteins (immunology), Protozoan Vaccines (administration & dosage), Protozoan Vaccines (immunology), Reactive Oxygen Species (metabolism), Recombinant Proteins (administration & dosage), Recombinant Proteins (immunology), Vaccines, Subunit (administration & dosage), Vaccines, Subunit (immunology), Vaccines, Synthetic (administration & dosage), Vaccines, Synthetic (immunology).
- MESH :
- chemical , administration & dosage : Protozoan Proteins, Protozoan Vaccines, Recombinant Proteins, Vaccines, Subunit, Vaccines, Synthetic.
- chemical , biosynthesis : Cytokines.
- chemical , immunology : Antibodies, Helminth, Antigens, Helminth, Immunoglobulin G, Immunoglobulin M, Protozoan Proteins, Protozoan Vaccines, Recombinant Proteins, Vaccines, Subunit, Vaccines, Synthetic.
- immunology : Brugia malayi, Elephantiasis, Filarial, Larva, Lymphocyte Activation.
- chemical , metabolism : Reactive Oxygen Species.
- parasitology : Elephantiasis, Filarial, Murinae.
- prevention & control : Elephantiasis, Filarial.
- Animals, Immunization.
Abstract
Although recombinant vaccines have several advantages over conventional vaccines, protection induced by single antigen vaccines is often inadequate for a multicellular helminth parasite. Therefore, immunoprophylactic efficacy of cocktail antigen vaccines comprised of several combinations of three Brugia malayi recombinant proteins BmAF-Myo, Bm-iPGM and Bm-TPP were evaluated. Myosin+TPP and iPGM+TPP provided the best protection upon B. malayi infective larval challenge with ∼70% reduction in adult worm establishment over non-vaccinated animals that was significantly higher than the protection achieved by any single antigen vaccine. Myosin+iPGM, in contrast did not provide any enhance protection over the single recombinant protein vaccines. Specific IgG, IgM level, IgG antibody subclasses levels (IgG1, IgG2a, IgG2b, IgG3), lymphocyte proliferation, reactive oxygen species level and cytokines level were also determined to elucidate the characteristics of the protective immune responses. Thus the study undertaken provided more insight into the cocktail vaccination approach to combat LF.
DOI: 10.1016/j.cimid.2013.05.003
PubMed: 23829972
Affiliations:
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(immunology)</term><term>Protozoan Vaccines (administration & dosage)</term><term>Protozoan Vaccines (immunology)</term><term>Reactive Oxygen Species (metabolism)</term><term>Recombinant Proteins (administration & dosage)</term><term>Recombinant Proteins (immunology)</term><term>Vaccines, Subunit (administration & dosage)</term><term>Vaccines, Subunit (immunology)</term><term>Vaccines, Synthetic (administration & dosage)</term><term>Vaccines, Synthetic (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes (immunologie)</term><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Antigènes d'helminthe (immunologie)</term><term>Brugia malayi (immunologie)</term><term>Cytokines (biosynthèse)</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Filariose lymphatique ()</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique 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lymphocytes</term><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Filariose lymphatique</term><term>Immunoglobuline G</term><term>Immunoglobuline M</term><term>Larve</term><term>Protéines de protozoaire</term><term>Protéines recombinantes</term><term>Vaccins antiprotozoaires</term><term>Vaccins sous-unitaires</term><term>Vaccins synthétiques</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Elephantiasis, Filarial</term><term>Larva</term><term>Lymphocyte Activation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Espèces réactives de l'oxygène</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term><term>Murinae</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Murinae</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Immunization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Filariose lymphatique</term><term>Immunisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although recombinant vaccines have several advantages over conventional vaccines, protection induced by single antigen vaccines is often inadequate for a multicellular helminth parasite. Therefore, immunoprophylactic efficacy of cocktail antigen vaccines comprised of several combinations of three Brugia malayi recombinant proteins BmAF-Myo, Bm-iPGM and Bm-TPP were evaluated. Myosin+TPP and iPGM+TPP provided the best protection upon B. malayi infective larval challenge with ∼70% reduction in adult worm establishment over non-vaccinated animals that was significantly higher than the protection achieved by any single antigen vaccine. Myosin+iPGM, in contrast did not provide any enhance protection over the single recombinant protein vaccines. Specific IgG, IgM level, IgG antibody subclasses levels (IgG1, IgG2a, IgG2b, IgG3), lymphocyte proliferation, reactive oxygen species level and cytokines level were also determined to elucidate the characteristics of the protective immune responses. Thus the study undertaken provided more insight into the cocktail vaccination approach to combat LF.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Kushwaha, Susheela" sort="Kushwaha, Susheela" uniqKey="Kushwaha S" first="Susheela" last="Kushwaha">Susheela Kushwaha</name><name sortKey="Misra Bhattacharya, Shailja" sort="Misra Bhattacharya, Shailja" uniqKey="Misra Bhattacharya S" first="Shailja" last="Misra-Bhattacharya">Shailja Misra-Bhattacharya</name><name sortKey="Nag, Jeetendra Kumar" sort="Nag, Jeetendra Kumar" uniqKey="Nag J" first="Jeetendra Kumar" last="Nag">Jeetendra Kumar Nag</name><name sortKey="Singh, Prashant Kumar" sort="Singh, Prashant Kumar" uniqKey="Singh P" first="Prashant Kumar" last="Singh">Prashant Kumar Singh</name></noCountry><country name="Inde"><noRegion><name sortKey="Shrivastava, Nidhi" sort="Shrivastava, Nidhi" uniqKey="Shrivastava N" first="Nidhi" last="Shrivastava">Nidhi Shrivastava</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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